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KMID : 1005220100250030141
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Journal of Embryo Transfer
2010 Volume.25 No. 3 p.141 ~ p.144
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Effect of In Vivo Embryo Production and Embryo Transfer Following Superovulation in Hanwoo
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Cho Sang-Rae
Choi Sun-Ho
Choe Chang-Yong
Son Jun-Kyu
Choi Soo-Ho
Kim Young-Joo
Lee Poong-Yeon
Yeon Seong-Heum
Kim Hyun-Jong
Son Dong-Soo
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Abstract
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In vivo embryo produced from Hanwoo donor cows were collected and transferred to Hanwoo recipients. Cows, at random stages of the estrous cycle, received Progesterone Releasing Intravaginal Device (CIDR-plus, InterAg, New Zealand) together with injection of 1 mg estradiol benzoate and 50 mg progesterone, and gonadotropin treatment began 4 day later. For superovulation, a total of 28 mg FSH was intramuscularly injected twice a day in the way of decreasing doses 4 day (5, 5, 4, 4, 3, 3, 2 and 2 mg). Twenty one Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered 7 days after the second insemination by flushing the uterus with Embryo Collection Medium. The results obtained were as follows: The rates of transferable embryos were 50.3%, and 78 fresh embryos at morulae and blastocysts stage were transferred into Hanwoo recipients on day 7 of estrus cycle. The pregnancy rates were first embryo transfer 55.6%, 2nd 62.9% and 3rd 57.9%, respectively. In conclusion, These results suggest that CIDR-based superovulation protocol may be effectively used for production of superior Hanwoo embryos. Also, since it seems the condition of recipient cows greatly affect pregnancy rate, it is very important to evaluate recipient for effective cattle production.
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KEYWORD
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bovine, embryos transfer donor, recipient, super-ovulation
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